Difference between revisions of "Part:BBa K3040115"

Latest revision as of 16:34, 16 October 2019

pFadD_fadR binding site

pFadD promoter is one of the regulator in the enzymes of fatty acid biosynthesis in E. coli. It is composed of two fadR recognition sites, and one CRP binding site. The fatty acid metabolism system of E. coli is consist of many parts, fadD, fadL, fadR, fadA, etc. Interestingly, each enzyme in this family has its own different sequence of fadR recognition site in its promoter, which makes these promoter having different strength, yet can still be interchangeable for us to apply in our system. Based on this fact, we tried pFadD promoter to take place of the weak pFadBA promoter. With two fadR recognition sites native in pFadD, we assume a better result in the sensitivity and a lower leakage in our pFadD promoter. Yet, we further modified pFadD promoter by replacing its CRP binding site with a FadR recognition site of the sequence of pFadBA promoter.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 33
Illegal XbaI site found at 219
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 33
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 33
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 33
Illegal XbaI site found at 219
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 33
Illegal XbaI site found at 219
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3040115 short</partinfo>
-dsaf
+pFadD promoter is one of the regulator in the enzymes of fatty acid biosynthesis in E. coli. It is composed of two fadR recognition sites, and one CRP binding site. The fatty acid metabolism system of E. coli is consist of many parts, fadD, fadL, fadR, fadA, etc. Interestingly, each enzyme in this family has its own different sequence of fadR recognition site in its promoter, which makes these promoter having different strength, yet can still be interchangeable for us to apply in our system.
+Based on this fact, we tried pFadD promoter to take place of the weak pFadBA promoter. With two fadR recognition sites native in pFadD, we assume a better result in the sensitivity and a lower leakage in our pFadD promoter. Yet, we further modified pFadD promoter by replacing its CRP binding site with a FadR recognition site of the sequence of pFadBA promoter.
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