Difference between revisions of "Part:BBa K3040105"
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| − | + | It is pointed out by the previous iGEM team, UPF CRG Barcelona_2018 iGEM, that the native promoter pFadBA has great leakage and is a weak promoter that isn’t ideal enough for our system. Therefore, to enhance the sensitivity and reduce the leakage of the promoter pFadBA for making it more suitable for our system, we modified the native promoter pFadBA with a mutant in its consensus sequence of -10 and -35 region. | |
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here--> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | According to the previous research [1], there is improvement in expression for pFadBA with consensus sequence. Furthermore, the improved in expression portion enhance greatly if there’s a point of mutant in consensus sequence. Therefore, we designed our promoter pFadBA with a mutant in its consensus sequence of -10 and -35 region, to promote its strength. | ||
| + | |||
| + | [Ref] | ||
| + | The-Sequence-of-Spacers-between-the-Consensus-Sequences-Modulates-the-Strength-of-Prokaryotic-Promoters | ||
| + | |||
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Latest revision as of 15:55, 16 October 2019
pFadBA_NTHU
It is pointed out by the previous iGEM team, UPF CRG Barcelona_2018 iGEM, that the native promoter pFadBA has great leakage and is a weak promoter that isn’t ideal enough for our system. Therefore, to enhance the sensitivity and reduce the leakage of the promoter pFadBA for making it more suitable for our system, we modified the native promoter pFadBA with a mutant in its consensus sequence of -10 and -35 region.
Usage and Biology
According to the previous research [1], there is improvement in expression for pFadBA with consensus sequence. Furthermore, the improved in expression portion enhance greatly if there’s a point of mutant in consensus sequence. Therefore, we designed our promoter pFadBA with a mutant in its consensus sequence of -10 and -35 region, to promote its strength.
[Ref] The-Sequence-of-Spacers-between-the-Consensus-Sequences-Modulates-the-Strength-of-Prokaryotic-Promoters
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]