Difference between revisions of "Part:BBa K3096007"
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This is a part consisting of all cofactors necessary for the functionality of the CRISPR/Cas3 system. It works if utilised with BBa_K3096001 and the respective CRISPR arrays targeting the to-be-degraded sequences. | This is a part consisting of all cofactors necessary for the functionality of the CRISPR/Cas3 system. It works if utilised with BBa_K3096001 and the respective CRISPR arrays targeting the to-be-degraded sequences. | ||
| − | ===Usage | + | ===Usage and Biology=== |
In the type I CISPR/Cas system the crRNA guides the surveillance complex Cascade ABCDE to | In the type I CISPR/Cas system the crRNA guides the surveillance complex Cascade ABCDE to | ||
complementary DNA sequence. The DNA is unwound, generating the so-called R-loop. The Cas3 helicase-nuclease protein attaches to this R-loop and unidirectionally degrades the DNA. Hereby, the large Cas3/Cascade complex pulls the substrate DNA towards itself, introducing single strand breaks. | complementary DNA sequence. The DNA is unwound, generating the so-called R-loop. The Cas3 helicase-nuclease protein attaches to this R-loop and unidirectionally degrades the DNA. Hereby, the large Cas3/Cascade complex pulls the substrate DNA towards itself, introducing single strand breaks. | ||
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==References== | ==References== | ||
| − | # | + | #'''Xia, Peng-Fei, et al'''. "Synthetic genetic circuits for programmable biological functionalities." ''Biotechnology advances'' ('''2019'''). |
| + | #'''Terns, Michael P., and Rebecca M. Terns'''. "CRISPR-based adaptive immune systems." ''Current opinion in microbiology'' 14.3 ('''2011'''): 321-327. | ||
Latest revision as of 07:13, 21 October 2020
Type I CRISPR/Cas system surveillance complex Cascade ABCDE
This is a part consisting of all cofactors necessary for the functionality of the CRISPR/Cas3 system. It works if utilised with BBa_K3096001 and the respective CRISPR arrays targeting the to-be-degraded sequences.
Usage and Biology
In the type I CISPR/Cas system the crRNA guides the surveillance complex Cascade ABCDE to complementary DNA sequence. The DNA is unwound, generating the so-called R-loop. The Cas3 helicase-nuclease protein attaches to this R-loop and unidirectionally degrades the DNA. Hereby, the large Cas3/Cascade complex pulls the substrate DNA towards itself, introducing single strand breaks.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- Xia, Peng-Fei, et al. "Synthetic genetic circuits for programmable biological functionalities." Biotechnology advances (2019).
- Terns, Michael P., and Rebecca M. Terns. "CRISPR-based adaptive immune systems." Current opinion in microbiology 14.3 (2011): 321-327.