Difference between revisions of "Part:BBa K2969012"

 
 
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An engineered CI transcription factor. We insert the HRV3C protease recognition sequence between N-terminal and C-terminal of CI. Because the structure of CI has been characterized, the function of the fragment between N-terminal and C-terminal is linking. So, we choose this inserting site to avoid bigger influence. In our project, this kind of CI can be cleaved by HRV3C so that it will lose activity.
 
An engineered CI transcription factor. We insert the HRV3C protease recognition sequence between N-terminal and C-terminal of CI. Because the structure of CI has been characterized, the function of the fragment between N-terminal and C-terminal is linking. So, we choose this inserting site to avoid bigger influence. In our project, this kind of CI can be cleaved by HRV3C so that it will lose activity.
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Latest revision as of 17:46, 19 October 2019


CI-HRV3Csite

An engineered CI transcription factor. We insert the HRV3C protease recognition sequence between N-terminal and C-terminal of CI. Because the structure of CI has been characterized, the function of the fragment between N-terminal and C-terminal is linking. So, we choose this inserting site to avoid bigger influence. In our project, this kind of CI can be cleaved by HRV3C so that it will lose activity.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]