Difference between revisions of "Part:BBa K2999004"
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<partinfo>BBa_K2999004 short</partinfo> | <partinfo>BBa_K2999004 short</partinfo> | ||
− | This part is a promoter which is regulated by the quorum sensing system in Pseudomonas aeruginosa, and the signal molecule which regulates the promoter is N-(butyry1)-1-homoserine lactone ( | + | This part is a promoter which is regulated by the quorum sensing system in ''Pseudomonas aeruginosa'', and the signal molecule which regulates the promoter is N-(butyry1)-1-homoserine lactone (BHL). |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | BHL and RhlR are self-inducing signaling molecules and receptor pairs for the <i>P. aeruginosa</i> Quorum sensing. When BHL and RhlR form a complex, they can induce downstream gene transcription.When our team knocks out rhlR gene and rhlI gene separately, <i>PA2069</i> promoter The enzyme activity was significantly reduced, so the <i>PA2069</i> promoter was transformed in response to BHL-RhlR. | ||
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+ | [[File:T--YAU-China--iGEM2019-BBa -6.png|500px|thumb|none|Figure 1.Determination of β - galactosidase activity of PA2069 promoter ]] | ||
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Latest revision as of 17:22, 21 October 2019
PA2069 Promoter
This part is a promoter which is regulated by the quorum sensing system in Pseudomonas aeruginosa, and the signal molecule which regulates the promoter is N-(butyry1)-1-homoserine lactone (BHL).
Usage and Biology
BHL and RhlR are self-inducing signaling molecules and receptor pairs for the P. aeruginosa Quorum sensing. When BHL and RhlR form a complex, they can induce downstream gene transcription.When our team knocks out rhlR gene and rhlI gene separately, PA2069 promoter The enzyme activity was significantly reduced, so the PA2069 promoter was transformed in response to BHL-RhlR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 365
Illegal XhoI site found at 698 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 96
Illegal NgoMIV site found at 271
Illegal NgoMIV site found at 297
Illegal NgoMIV site found at 482
Illegal NgoMIV site found at 602 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 989