Difference between revisions of "Part:BBa K2926058"

 
 
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<partinfo>BBa_K2926058 short</partinfo>
 
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To visualize the interactions of the n-terminal lectin binding domain of the S. cerevisiae protein Flo11 we fused the coding sequence n-terminally to the coding sequence of the fluorescence reporter protein mCherry. mCherry was n-terminally fused to the M13 bacteriophage major coat protein pVIII. The whole protein was fused to a c-terminal hexahistidine tag to enable protein purification via metal ions.  
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To visualize the interactions of the n-terminal lectin binding domain of the <i>S. cerevisiae</i> protein Flo11 we fused the coding sequence N-terminally to the coding sequence of the fluorescence reporter protein mCherry. mCherry was N-terminally fused to the M13 bacteriophage major coat protein pVIII. The whole protein was fused to a C-terminal hexahistidine tag to enable protein purification via metal ions.  
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h1>Sequence and Features</h1></span>
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Sequence was validated by Sanger sequencing.
 
<partinfo>BBa_K2926058 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2926058 SequenceAndFeatures</partinfo>
  

Latest revision as of 21:53, 17 October 2019


Fusion protein of Flo11 (yeast), mCherry and pVIII (M13 bacteriophage) with purification tag To visualize the interactions of the n-terminal lectin binding domain of the S. cerevisiae protein Flo11 we fused the coding sequence N-terminally to the coding sequence of the fluorescence reporter protein mCherry. mCherry was N-terminally fused to the M13 bacteriophage major coat protein pVIII. The whole protein was fused to a C-terminal hexahistidine tag to enable protein purification via metal ions.

Sequence and Features

Sequence was validated by Sanger sequencing.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]