Difference between revisions of "Part:BBa K2999001"
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<partinfo>BBa_K2999001 short</partinfo> | <partinfo>BBa_K2999001 short</partinfo> | ||
− | This part is mainly used | + | This part is mainly used to encode the extracellular polysaccharide hydrolase PslG and the hydrolysis of such extracellular polysaccharides of biofilm produced by ''Pseudomonas aeruginosa'' PAO1 . The first 50 bp of the sequence is its ribosome binding site. |
− | For compatibility, 254 nucleotide G was changed to A (same as leucine) and 1358 nucleotide G to A (same as leucine). | + | For compatibility, 254<sup>th</sup> nucleotide G was changed to A (same as leucine) and 1358<sup>th</sup> nucleotide G to A (same as leucine). |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | Please click:< | + | Please click:<html><a href='https://parts.igem.org/Part:BBa_K2999009'>BBa_K2999009</a></html> |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 14:32, 21 October 2019
RBS+PslG
This part is mainly used to encode the extracellular polysaccharide hydrolase PslG and the hydrolysis of such extracellular polysaccharides of biofilm produced by Pseudomonas aeruginosa PAO1 . The first 50 bp of the sequence is its ribosome binding site. For compatibility, 254th nucleotide G was changed to A (same as leucine) and 1358th nucleotide G to A (same as leucine).
Usage and Biology
Please click:BBa_K2999009
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 356
Illegal XhoI site found at 360 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 660
Illegal NgoMIV site found at 849
Illegal NgoMIV site found at 857 - 1000COMPATIBLE WITH RFC[1000]