Difference between revisions of "Part:BBa K3187026"
 
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<html>
 
<html>
 
<p>
 
<p>
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<h1>Profile</h1>
 
<table style=“width:80%“>
 
<table style=“width:80%“>
 
<tr>
 
<tr>
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<td>name</td>
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<td><b>name</b></td>
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<td>coat protein with LPETGG in pET24</td>
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<td>mCherry</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td>base pairs</td>
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<td><b>base pairs</b></td>
 
<td>705</td>
 
<td>705</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td>molecular weight</td>
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<td><b>molecular weight</b></td>
 
<td>26.6 kDa</td>
 
<td>26.6 kDa</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td>origin</td>
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<td><b>origin</b></td>
 
<td>synthetic, derived from <i>Discosoma</i> sp.</td>
 
<td>synthetic, derived from <i>Discosoma</i> sp.</td>
 
</tr>
 
</tr>
 
<tr>
 
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<td>properties</td>
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<td><b>properties</b></td>
 
<td> Red fluorescent, Ex λ: 587nm, Em λ: 610 nm </td>
 
<td> Red fluorescent, Ex λ: 587nm, Em λ: 610 nm </td>
 
</tr>
 
</tr>
 
</table>
 
</table>
 
<br>
 
<br>
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<h1>Usage and Biology</h1>
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<p>
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mCherry is a red fluorescent protein, which is derived from <i>Discosoma</i> sp.. It absorbs light with a wavelength of 587 nm and emits light with a wavelength of 610 nm. mCherry can be modified N- and C-terminal without a deficit in fluorescent.<sup id="cite_ref-1" class="reference"><a href="#cite_note-1">[1] </a> </sup><br>
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</p>
  
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mCherry is a red fluorescent protein, which is derived from <i>Discosoma</i> sp.. It absorbs light with a wavelength of 587 nm and emits light with a wavelength of 610 nm. mCherry can be modified N- and C-terminal without a deficit in fluorescent.<sup id="cite_ref-1" class="reference"><a href="#cite_note-1">[1] </a> </sup>
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https://2019.igem.org/wiki/images/c/c6/T--TU_Darmstadt--Registry_mCherry.jpg
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            <div class="caption">
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                <p>
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                    <b>Figure 1:</b>
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                    Crystal structure of mCherry.
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                </p>
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            </div>
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<br>
 
<br>
 
<h2>References</h2>
 
<h2>References</h2>
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<partinfo>BBa_K3187026 parameters</partinfo>
 
<partinfo>BBa_K3187026 parameters</partinfo>
 
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<h2>References</h2>
 
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            <ol class="references">
 
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                <li id="cite_note-1">
 
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    <span class="mw-cite-backlink">
 
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      <a href="#cite_ref-1">↑</a>
 
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    </span>
 
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                    <span class="reference-text">
 
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Nathan Shaner, Robert Campbell, Paul Steinbach, Ben Giepmans, Amy Palmer and Roger Tsien, Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein, Nature Biotechnology, 2004, 22: 1567-1572
 
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      <a rel="nofollow" class="external autonumber" href="#https://www.nature.com/articles/nbt1037">[1] </a>
 
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    </span>
 
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                </li>
 

Latest revision as of 16:27, 21 October 2019


mCherry Fluorescence Protein

Profile

name mCherry
base pairs 705
molecular weight 26.6 kDa
origin synthetic, derived from Discosoma sp.
properties Red fluorescent, Ex λ: 587nm, Em λ: 610 nm

Usage and Biology

mCherry is a red fluorescent protein, which is derived from Discosoma sp.. It absorbs light with a wavelength of 587 nm and emits light with a wavelength of 610 nm. mCherry can be modified N- and C-terminal without a deficit in fluorescent.[1]


References

  1. ↑ Nathan Shaner, Robert Campbell, Paul Steinbach, Ben Giepmans, Amy Palmer and Roger Tsien, Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein, Nature Biotechnology, 2004, 22: 1567-1572 [1]

    2. Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      COMPATIBLE WITH RFC[25]
    • 1000
      COMPATIBLE WITH RFC[1000]