Difference between revisions of "Part:BBa K3239007"
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<partinfo>BBa_K3239007 short</partinfo>
 
<partinfo>BBa_K3239007 short</partinfo>
  
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yEFGP3 reporter gene expressed by pAOX1
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''yEFGP3'' reporter gene expressed by ''P<sub>AOX1''.
  
 
===Usage and Biology===
 
===Usage and Biology===
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This construct is the control used in our experiment. After homologous recombination into wild type P. pastoris, the EGFP expressed shall serve as an indicator of AOX1 expression levels. Note that the original pAOX1-AOX1 copy remains intact after the recombination.  
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This construct is the control used in our experiment. After homologous recombination into wild type ''P. pastoris'', the EGFP protein expressed shall serve as an indicator of ''AOX1'' expression levels. Note that the original ''P<sub>AOX1''-''AOX1'' copy remains intact after the recombination.  
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[[File: pAOX1-GFP.png|400px|thumb|Figure 1. Illustration of the ''P<sub>AOX1''-GFP construct]]
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===Experience===
 
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we labeled our strains containing this construct AOX1-GFP and used it as the positive control of our data.
 
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The following data are acquired after 108h YNM (1.34% yeat nitrogenous base with 0.5% methanol, 50mg/mL histidine and 10mg/mL biotin) incubation, sampled every 12h.
 
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Methanol is added to the media every 24 hours to maintain the methanol concentration.
 
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Three parallels are performed and the data shown is the average of the three parallels.
 
  
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[[File:Methanol(24h)AOX1-GFP.png|400px|thumb|left|Figure 1. AOX1-GFP methanol consumption curve]]
 
  
  
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===Source===
 
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Both the promoter and the reporter are registered.
 
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pAOX1: BBa_K3239003
 
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yEGFP3: BBa_K3239006
 
  
 
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Latest revision as of 06:22, 18 October 2019


pAOX1-GFP

yEFGP3 reporter gene expressed by PAOX1.

Usage and Biology

This construct is the control used in our experiment. After homologous recombination into wild type P. pastoris, the EGFP protein expressed shall serve as an indicator of AOX1 expression levels. Note that the original PAOX1-AOX1 copy remains intact after the recombination.

Figure 1. Illustration of the PAOX1-GFP construct








Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 935
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]