Difference between revisions of "Part:BBa K3176020:Design"

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===Design Notes===
 
===Design Notes===
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We had to deal with the sequencing of our aptamer candidates and select the one with the highest affinity and specificity for E. faecium.  
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Sequence was obtained by Eurofins Genomics after the sequencing of bacterial clones.
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===Source===
 
===Source===
  
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BBa_K3176020 was made from a DNA library synthesized by Eurofins Genomics and selected using the Whole-cell SELEX method.
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Made from a DNA library synthesized by Eurofins Genomics, we selected this DNA aptamer using the Whole-cell SELEX method.
  
 
===References===
 
===References===
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Campbell, R. E., et al. (2002). A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America, 99(12), 7877–7882. doi:10.1073/pnas.082243699
 
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Falord, M., et al. (2011). Investigation of the Staphylococcus aureus GraSR regulon reveals novel links to virulence, stress response and cell wall signal transduction pathways. PloS one, 6(7), e21323. doi:10.1371/journal.pone.0021323
 
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Aucher, W. et al. (2004). Differences in mesentericin secretion systems from two Leuconostoc strains. FEMS Microbiology Letters, 232(1), pp. 15–22. doi: 10.1016/S0378-1097(04)00011-4.
 

Latest revision as of 23:01, 21 October 2019


Specific aptamer for E. faecium


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was obtained by Eurofins Genomics after the sequencing of bacterial clones.

Source

Made from a DNA library synthesized by Eurofins Genomics, we selected this DNA aptamer using the Whole-cell SELEX method.

References