Difference between revisions of "Part:BBa K3239006"

 
 
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<partinfo>BBa_K3239006 short</partinfo>
 
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reporter EGFP variant optimized for yeast for FACS
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A yeast- and FACS-optimized ''EGFP'' variant.
  
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===Usage and Biology===
 
===Usage and Biology===
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This ''yEGFP3'' reporter gene is an ''EGFP'' variant optimized for yeast and FACS cell sorting.
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===Functional Parameters===
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yEGFP3 protein is reported to have a half-life of about 7h in ''Saccharomyces cerevisiae'' (Mateus et al., 2000), the budding yeast to which ''Pichia pastoris'' is very similar.
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A single-base mutation is introduced for Type IIS compatibility. This single mutation does not affect the protein sequence.
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===Reference===
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Protein: https://www.ncbi.nlm.nih.gov/protein/82569482
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Source: https://www.ncbi.nlm.nih.gov/nuccore/82569479
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Mateus, C., & Avery, S. V. (2000). Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast, 16(14), 1313–1323. doi: 10.1002/1097-0061(200010)16:14<1313::aid-yea626>3.0.co;2-o
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===Functional Parameters===
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<partinfo>BBa_K3239006 parameters</partinfo>
 
<partinfo>BBa_K3239006 parameters</partinfo>
 
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Latest revision as of 06:13, 18 October 2019


yEGFP3

A yeast- and FACS-optimized EGFP variant.

Usage and Biology

This yEGFP3 reporter gene is an EGFP variant optimized for yeast and FACS cell sorting.

Functional Parameters

yEGFP3 protein is reported to have a half-life of about 7h in Saccharomyces cerevisiae (Mateus et al., 2000), the budding yeast to which Pichia pastoris is very similar.

A single-base mutation is introduced for Type IIS compatibility. This single mutation does not affect the protein sequence.

Reference

Protein: https://www.ncbi.nlm.nih.gov/protein/82569482

Source: https://www.ncbi.nlm.nih.gov/nuccore/82569479

Mateus, C., & Avery, S. V. (2000). Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast, 16(14), 1313–1323. doi: 10.1002/1097-0061(200010)16:14<1313::aid-yea626>3.0.co;2-o

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]