Difference between revisions of "Part:BBa K3239000"

Latest revision as of 05:40, 18 October 2019

Promoter for the Prm1 transcription factor in the P. pastoris GS115 strain

Promoter for the PRM1 gene in the P. pastoris GS115 strain. Upregulated by Prm1 and downregulated by Mit1.

Usage and Biology

The PRM1 promoter (P_PRM1) is homogeneous to the P. pastoris GS115 strain. It is self-upregulated by the Prm1 protein and downregulated by the Mit1 protein, both transcription factors homogeneous to the P. pastoris GS115 strain.

Functional Parameters

Published literature has shown that this promoter is strongly induced by methanol but is relatively leaky compared to the MIT1 promoter (P_MIT1) and the AOX1 promoter (P_AOX1).

Reference

Liang, S., Wang, B., Pan, L., Ye, Y., He, M., Han, S., … Lin, Y. (2012). Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing. BMC Genomics, 13(1). https://doi.org/10.1186/1471-2164-13-738

Shi, L., Wang, J., Wang, X., Zhang, Y., Song, Z., Cai, M., & Zhou, X. (2019). Transcriptome and metabolome analyses reveal global behaviour of a genetically engineered methanol-independent Pichia pastoris strain. Process Biochemistry, 76(August 2018), 46–54. https://doi.org/10.1016/j.procbio.2018.10.014

Wang, X., Wang, Q., Wang, J., Zhou, M., Shi, L., Zhou, X., … Shen, W. (2016). Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 ( AOX1 ) Promoter in Pichia pastoris. Journal of Biological Chemistry, 291(12), 6245–6261. https://doi.org/10.1074/jbc.m115.692053

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 293
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3239000 short</partinfo>
-The promoter of the PRM1 gene in wildtype P. pastoris. Upregulated by Prm1 and downregulated by Mit1.
+Promoter for the ''PRM1'' gene in the ''P. pastoris GS115'' strain. Upregulated by Prm1 and downregulated by Mit1.
 ===Usage and Biology===
-The PRM1 promoter is homogenous to the P. pastoris GS115 strain and is critical in the regulatory pathway of the expression of alcohol oxidase 1 (AOX1).
+The ''PRM1'' promoter (''P<sub>PRM1'') is homogeneous to the ''P. pastoris GS115'' strain. It is self-upregulated by the Prm1 protein and downregulated by the Mit1 protein, both transcription factors homogeneous to the ''P. pastoris GS115'' strain.
-Prm1 is one of the three critical transcription factors that upregulate the activity of the AOX1 promoter. When methanol is present, the activity of the PRM1 promoter is upregulated, and further amplified by the self-upregulation of Prm1 (Prm1 upregulates PRM1 promoter).
 ===Functional Parameters===
+Published literature has shown that this promoter is strongly induced by methanol but is relatively leaky compared to the ''MIT1 promoter'' (''P<sub>MIT1'') and the ''AOX1'' promoter (''P<sub>AOX1'').
+===Reference===
+Liang, S., Wang, B., Pan, L., Ye, Y., He, M., Han, S., … Lin, Y. (2012). Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing. BMC Genomics, 13(1). https://doi.org/10.1186/1471-2164-13-738
+Shi, L., Wang, J., Wang, X., Zhang, Y., Song, Z., Cai, M., & Zhou, X. (2019). Transcriptome and metabolome analyses reveal global behaviour of a genetically engineered methanol-independent Pichia pastoris strain. Process Biochemistry, 76(August 2018), 46–54. https://doi.org/10.1016/j.procbio.2018.10.014
+Wang, X., Wang, Q., Wang, J., Zhou, M., Shi, L., Zhou, X., … Shen, W. (2016). Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 ( AOX1 ) Promoter in Pichia pastoris. Journal of Biological Chemistry, 291(12), 6245–6261. https://doi.org/10.1074/jbc.m115.692053