Difference between revisions of "Part:BBa K2936007"
 
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An alpha peptide chain encoded by a gene LacZ
 
An alpha peptide chain encoded by a gene LacZ
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===Description===
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Short description: Reporter gene.<br>
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The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) ).
  
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===Source===
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<i> E. coli </i>
  
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===<h1>Usage and Biology</h1>===
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===Design consideration===
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<p>
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  The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) )
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</p>
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===<h1>Characterize</h1>===
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<p>
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LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
 
LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
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<br>Mechanism:
 
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  <img src="https://2019.igem.org/File:T--ZJUT-China--igem-part07jili.png">
 
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</html>
 
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</p>
 
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===<h1>Experimental Results</h1>===
 
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<p>Form 1 The absorbance of different reaction solution at the wavelength of350 nm</p>
 
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  <img src="https://2019.igem.org/File:T--ZJUT-China--igem-part07form.png">
 
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</html>
 
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<html>
 
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  <img src="https://2019.igem.org/File:T--ZJUT-China--igem-part07reaction.jpg">
 
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</html>
 
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<p>Figure 7.1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.</p>
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K2936007 SequenceAndFeatures</partinfo>
 
  
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===Mechanism===
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https://static.igem.org/mediawiki/parts/d/d6/T--ZJUT-China--igem-part01fanying.png
  
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===Experiment===
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Form 7.1 The absorbance of different reaction solution at the wavelength of 350 nm<br>
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https://static.igem.org/mediawiki/parts/5/5a/T--ZJUT-China--igem-part07form.png<br>
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<html>
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<img src="https://static.igem.org/mediawiki/parts/4/4e/T--ZJUT-China--igem-1234.png" width="538px"></html><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Figure 7.1 The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right
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===Analysis===
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From the figure 7.1, the color of the third group is enough obvious compared to the background. And at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD600=0.5) to TMB is 1 to 10 is the most ideal ratio.
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===Reference===
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[1]Josefine Liljeruhm, Saskia K. Funk, Sandra Tietscher, Anders D. Edlund, (2018) Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. [J]. Biological Engineering (2018) 12:8<br>
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2936007 parameters</partinfo>
 
<partinfo>BBa_K2936007 parameters</partinfo>
 
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Latest revision as of 06:49, 19 October 2019


LacZ alpha

An alpha peptide chain encoded by a gene LacZ

Description

Short description: Reporter gene.
The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) ).

Source

E. coli

Design consideration

LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.

Mechanism

T--ZJUT-China--igem-part01fanying.png

Experiment

Form 7.1 The absorbance of different reaction solution at the wavelength of 350 nm
T--ZJUT-China--igem-part07form.png

          Figure 7.1 The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right

Analysis

From the figure 7.1, the color of the third group is enough obvious compared to the background. And at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD600=0.5) to TMB is 1 to 10 is the most ideal ratio.

Reference

[1]Josefine Liljeruhm, Saskia K. Funk, Sandra Tietscher, Anders D. Edlund, (2018) Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. [J]. Biological Engineering (2018) 12:8