Difference between revisions of "Part:BBa K3110044:Design"

 
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===References===
 
===References===
 +
iGEM ETH zurich 2015<br>
 +
iGEM NUS Singapore 2016<br>

Latest revision as of 16:00, 21 October 2019


J23117 Medium RBS sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 67


Design Notes

None.


Source

sfGFP was obtained from the Biobrick. J23117+B0032 were obtained as gBlocks from IDT and these were joined together using SOEing (Spliced Overlap Extenstion) PCR.


References

iGEM ETH zurich 2015
iGEM NUS Singapore 2016