Difference between revisions of "Part:BBa K3110033"

 
 
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<partinfo>BBa_K3110033 short</partinfo>
 
<partinfo>BBa_K3110033 short</partinfo>
  
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L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) and the regulator element (lldR) under the control of a strong promoter and a weak RBS.
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This construct has L-Lactate Permease (lldP), Regulator element (lldR) and L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and weak RBS . lldP permits the entry of L-Lactate inside the bacterial cell, lldR has the dual role of an activator and a repressor and  lldD cleaves L-Lactate to pyruvate . Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldP, lldR and lldD by varying  the strength of the promoter and RBS controlling their production.
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<partinfo>BBa_K3110033 parameters</partinfo>
 
<partinfo>BBa_K3110033 parameters</partinfo>
 
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===Characterisation===
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Due to insufficient time, the construct could not be characterised.

Latest revision as of 17:03, 21 October 2019


Strong Promoter Weak RBS lldP+lldR+lldD

This construct has L-Lactate Permease (lldP), Regulator element (lldR) and L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and weak RBS . lldP permits the entry of L-Lactate inside the bacterial cell, lldR has the dual role of an activator and a repressor and lldD cleaves L-Lactate to pyruvate . Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldP, lldR and lldD by varying the strength of the promoter and RBS controlling their production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3649
    Illegal AgeI site found at 463
    Illegal AgeI site found at 926
    Illegal AgeI site found at 1364
    Illegal AgeI site found at 1523
    Illegal AgeI site found at 1535
    Illegal AgeI site found at 2281
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3086


Characterisation

Due to insufficient time, the construct could not be characterised.