Difference between revisions of "Part:BBa K2943901"
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<partinfo>BBa_K2943901 short</partinfo> | <partinfo>BBa_K2943901 short</partinfo> | ||
| − | + | This sequence is part of a composite part (BBa_K2943902) that aims to improve an existing composite part (BBa_K608014). The sequence is based on the monomer RFP (mRFP, BBa_E1010) with several mutations inside to optimize expression in our composite part. Mutations were placed in order to optimize the mRNA folding energy (calculated by VienneRNA) to gain more efficient translation initiation and therefore higher expression. | |
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Latest revision as of 22:24, 21 October 2019
Improved mRFP
This sequence is part of a composite part (BBa_K2943902) that aims to improve an existing composite part (BBa_K608014). The sequence is based on the monomer RFP (mRFP, BBa_E1010) with several mutations inside to optimize expression in our composite part. Mutations were placed in order to optimize the mRNA folding energy (calculated by VienneRNA) to gain more efficient translation initiation and therefore higher expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]