Difference between revisions of "Part:BBa K143009:Design"
m (→References) |
m |
||
Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
− | ===<big>'''3' Integration sequence for the | + | ===<big>'''3' Integration sequence for the pyrD locus of ''B.subtilis'''''</big>=== |
<br> | <br> | ||
Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
− | The | + | The pyrD integration sequences were based upon the pyrD gene sequence [1] and the sequence of the upstream region on the chromosome (found using NCBI's sequence viewer). The upstream and pyrD gene sequence was analysed for restriction sites and primers (with biobrick prefix and suffix sequences) for two approximately equally sized integration sequences were desgined. The integration sequences were synthesised by Geneart |
===Source=== | ===Source=== | ||
− | The 3’ integration sequence was taken from the ''B.subtilis'' chromosome and is homologous to the front section of the | + | The 3’ integration sequence was taken from the ''B.subtilis'' chromosome and is homologous to the front section of the pyrD gene. It was synthesised by Geneart |
===References=== | ===References=== |
Latest revision as of 23:02, 25 October 2008
3' Integration sequence for the pyrD locus of B.subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The pyrD integration sequences were based upon the pyrD gene sequence [1] and the sequence of the upstream region on the chromosome (found using NCBI's sequence viewer). The upstream and pyrD gene sequence was analysed for restriction sites and primers (with biobrick prefix and suffix sequences) for two approximately equally sized integration sequences were desgined. The integration sequences were synthesised by Geneart
Source
The 3’ integration sequence was taken from the B.subtilis chromosome and is homologous to the front section of the pyrD gene. It was synthesised by Geneart
References
<biblio>
- 1 pmid=9384377
</biblio>