Difference between revisions of "Part:BBa K143036:Design"

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<partinfo>BBa_K143036 short</partinfo>
 
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The wild-type XylR protein was identified using the complete genome sequene of B. subtilis <cite>#1</cite> and PCR primers designed from this sequence. Biobrick prefix and suffix standards were added to the XylR sequence and the gene cloned by PCR with Pfu DNA polymerase.
 
The wild-type XylR protein was identified using the complete genome sequene of B. subtilis <cite>#1</cite> and PCR primers designed from this sequence. Biobrick prefix and suffix standards were added to the XylR sequence and the gene cloned by PCR with Pfu DNA polymerase.
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====Template====
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''B. subtilis'' Chromosome
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*We thank Dr. Angelika Grundling, Centre for Molecular Microbiology and Infection, Imperial College for providing the chromosome preparation for our PCR
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====Forward Primer====
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5'- GCT CTA GAT GAC TGG ATT AAA TAA ATC AAC - 3'
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====Reverse Primer====
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5'- GGA CTA GTA TTA TTA CAT TGT AAT CAT GTC CAG -3'
  
 
===Source===
 
===Source===

Latest revision as of 00:23, 29 October 2008

Xylose operon regulatory protein



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The wild-type XylR protein was identified using the complete genome sequene of B. subtilis #1 and PCR primers designed from this sequence. Biobrick prefix and suffix standards were added to the XylR sequence and the gene cloned by PCR with Pfu DNA polymerase.

Template

B. subtilis Chromosome

  • We thank Dr. Angelika Grundling, Centre for Molecular Microbiology and Infection, Imperial College for providing the chromosome preparation for our PCR

Forward Primer

5'- GCT CTA GAT GAC TGG ATT AAA TAA ATC AAC - 3'

Reverse Primer

5'- GGA CTA GTA TTA TTA CAT TGT AAT CAT GTC CAG -3'

Source

The XylR protein was PCR cloned from the B. subtilis genome using Pfu DNA polymerase

References

<biblio>

  1. 1 pmid=9384377

</biblio>