Difference between revisions of "Part:BBa K143013:Design"

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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K143013 short</partinfo>
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===<big>Promoter 43 a constitutive promoter for ''B. subtilis''</big>===
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<br>
  
 
<partinfo>BBa_K143013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143013 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick standard was applied to the promoter P43 sequence.
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The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick prefix and suffix standard was applied to the promoter P43 sequence.
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===Source===
 
===Source===

Latest revision as of 13:32, 18 September 2008

Promoter 43 a constitutive promoter for B. subtilis



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick prefix and suffix standard was applied to the promoter P43 sequence.

Source

The part was designed using the sequence from the B.subtilis genome and from previously published papers 1. This sequence was then synthesised by Geneart.

References

<biblio>

  1. 1 pmid=6330116

</biblio>