Difference between revisions of "Part:BBa K143008:Design"

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<big>'''5' Integration sequence for the PyrD locus of ''B.subtilis'''''</big>
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__NOTOC__
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===<big>'''5' Integration sequence for the pyrD locus of ''B.subtilis'''''</big>===
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<br>
  
 
<partinfo>BBa_K143008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143008 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
  
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The PyrD integration sequences were designed from the PyrD gene's Genbank entry<cite>#1</cite> and identification of the sequence directly upstream of the gene on the chromosome (found using NCBI's sequence viewer). The upstream and PyrD gene sequence was analysed for restriction sites and primers (with biobrick prefix and suffix sequences) for two approximately equally sized integration sequences were desgined. The integration sequences were synthesised by Geneart
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The pyrD integration sequences were based upon the pyrD gene sequence <cite>#1</cite> and the sequence of the upstream region on the chromosome (found using NCBI's sequence viewer). The upstream and pyrD gene sequence was analysed for restriction sites and primers (with biobrick prefix and suffix sequences) for two approximately equally sized integration sequences were desgined. The integration sequences were synthesised by Geneart
  
 
===Source===
 
===Source===
  
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The 5’ integration sequence was taken from the ''B.subtilis'' chromosome and is homologous to the front section of the PyrD gene. It was synthesised by Geneart
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The 5’ integration sequence was taken from the ''B.subtilis'' chromosome and is homologous to the front section of the pyrD gene. It was synthesised by Geneart
  
 
===References===
 
===References===
  
 
<biblio>
 
<biblio>
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#1 http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene, Gene ID:938633
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#1 pmid=9384377
 
</biblio>
 
</biblio>

Latest revision as of 23:01, 25 October 2008

5' Integration sequence for the pyrD locus of B.subtilis



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 208

Design Notes

The pyrD integration sequences were based upon the pyrD gene sequence #1 and the sequence of the upstream region on the chromosome (found using NCBI's sequence viewer). The upstream and pyrD gene sequence was analysed for restriction sites and primers (with biobrick prefix and suffix sequences) for two approximately equally sized integration sequences were desgined. The integration sequences were synthesised by Geneart

Source

The 5’ integration sequence was taken from the B.subtilis chromosome and is homologous to the front section of the pyrD gene. It was synthesised by Geneart

References

<biblio>

  1. 1 pmid=9384377

</biblio>