Difference between revisions of "Part:BBa K143015"

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<partinfo>BBa_K143015 short</partinfo>
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===<big>Promoter hyper-spank for ''B. subtilis''</big>===
  
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Promoter hyper-spank is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter hyper-spank can be induced by addition of Isopropyl &#946;-D-1-thiogalactopyranoside (IPTG). The context with which we used the promoter hyper-spank, was to take an input of IPTG and give '''Polymerase Per Second'''(PoPS) as an output. IPTG does not induce the promoter hyper-spank directly, but requires the transcriptional regulator '''LacI''', (<bbpart>BBa_K413035</bbpart>). This means that LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank.
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Promoter hyper-spank is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl &#946;-D-1-thiogalactopyranoside (IPTG). The context with which we used the promoter hyper-spank, was to take an input of IPTG and give '''Polymerase Per Second'''(PoPS) as an output. IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator '''LacI''', (<bbpart>BBa_K143033</bbpart>).  
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'''LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank as an inducible promoter'''.
 
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<span class='h3bb'><big>'''Sequence and Features'''</big></span>
 
<span class='h3bb'><big>'''Sequence and Features'''</big></span>
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<partinfo>BBa_K143015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143015 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K143015 parameters</partinfo>
 
<partinfo>BBa_K143015 parameters</partinfo>
 
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===References===
 
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<biblio>
 
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#1 pmid=15028686
 
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#2 pmid=12169614
 
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#3 pmid=17720779
 
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</biblio>
 

Latest revision as of 14:10, 7 October 2008

Promoter hyper-spank for B. subtilis


Promoter hyper-spank is an inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG). The context with which we used the promoter hyper-spank, was to take an input of IPTG and give Polymerase Per Second(PoPS) as an output. IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator LacI, (BBa_K143033).

LacI must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]