Difference between revisions of "Part:BBa K3228042"

 
 
(2 intermediate revisions by one other user not shown)
Line 1: Line 1:
 +
<html>
 +
<div class="titleWrapper"><div class="titleBackground" style="background-image:none"></div><div class="title"></div></div>
 +
<article>
 +
<style>
 +
.imageContainer1x2 {
 +
display: flex;
 +
flex-wrap: wrap;
 +
justify-content: space-around;
 +
align-items: center;
 +
}
 +
.imageContainer1x2 div {
 +
width: 50%;
 +
margin-bottom: 2%;
 +
text-align:center;
 +
}
 +
.imageContainer1x2 div img {
 +
width: 100%;
 +
}
 +
.imageContainer1x4 {
 +
display: flex;
 +
flex-wrap: wrap;
 +
justify-content: space-around;
 +
align-items: center;
 +
}
 +
.imageContainer1x4 div {
 +
width: 23%;
 +
margin-bottom: 2%;
 +
text-align:center;
 +
}
 +
.imageContainer1x4 div img {
 +
width: 100%;
 +
}
 +
<i>
 +
</style>
 +
</html>
  
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3228042 short</partinfo>
 
<partinfo>BBa_K3228042 short</partinfo>
  
−
test
+
===Sequence and Features===
 +
<partinfo>BBa_K3228042 SequenceAndFeatures</partinfo>
  
−
<!-- Add more about the biology of this part here
+
===Description===
−
===Usage and Biology===
+
  
−
<!-- -->
+
<html>
−
<span class='h3bb'>Sequence and Features</span>
+
<p align="justify">
−
<partinfo>BBa_K3228042 SequenceAndFeatures</partinfo>
+
TeLuc is a triple mutant of NanoLuc. It bypasses the absorption of Chlorophyll A, making it the more suitable reporter for phototrophic organism.
 +
 
 +
<br>
 +
<br>
 +
This part is contained in a collection of reporters for <i>Synechococcus elongatus</i> UTEX2973 from <a href="https://2019.igem.org/Team:Marburg">iGEM Marburg 2019.</a> They are specifically chosen for cyanobacteria as they show a different absorption and excitation behaviour compared to other bacterial cells due to their photosystems. Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set should be seen as a recommendation of reporters based on
 +
<a href="https://2019.igem.org/Team:Marburg/Results">experimental data</a>and literature. They are either fluorescence reporters that bypass the wavelength at which cyanobacteria absorb and emit light or luminescent reporters that show a greater signal strength over background signal.
 +
 +
</p>
 +
</html>
 +
 
 +
===Compability===
 +
 
 +
<html>
 +
<p align="justify">
 +
 
 +
These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design"> Design of the Marburg Collection</a>.
 +
</p>
 +
</html>
 +
 
 +
===Results===
 +
 
 +
<html>
 +
<p align="justify">
 +
The Bioluminescence of nanoLuc in comparison to teLuc have been measured in e.Coli.
 +
 
 +
</p>
 +
</html>
 +
https://2019.igem.org/wiki/images/4/4f/T--Marburg--Measurement-TeLUC%2BNanoluc.png
 +
 
 +
===Available reporters===
 +
 
 +
<div class="PCListIcon" style="display:flex;flex-direction:row; flex-wrap: nowrap; justify-content:space-evenly; align-items:flex-start;font-size:82%;">
 +
 
 +
[[File:Icon L-Reporter.png|90px|thumb|none|
 +
 
 +
<html>
 +
<ul>
 +
<b>
 +
 
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228040">K3228040 </a> <br>(NanoLuc) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228041">K3228041 </a> <br>(NanoLuc S.e.)</li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228042">K3228042 </a> <br> (teLuc S.e.) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228043">K3228043 </a> <br> (Antares S.e.) </li>
 +
</b>
 +
</ul>
 +
</html>]]
  
 +
[[File:Icon F-Reporter.png|90px|thumb|none|
 +
<html>
 +
<ul>
 +
<b>
  
−
<!-- Uncomment this to enable Functional Parameter display
+
<li> <a href="https://parts.igem.org/Part:BBa_K3228044">K3228044 </a> <br> (eYFP S.e.) </li>
−
===Functional Parameters===
+
<li> <a href="https://parts.igem.org/Part:BBa_K3228045">K3228045 </a> <br> (phluorin2 S.e.) </li>
−
<partinfo>BBa_K3228042 parameters</partinfo>
+
<li> <a href="https://parts.igem.org/Part:BBa_K3228046">K3228046 </a> <br> (rxYFP S.e.) </li>
−
<!-- -->
+
<li> <a href="https://parts.igem.org/Part:BBa_K3228047">K3228047 </a> <br> (sfGFP S.e.) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228048">K3228048 </a> <br> (sfGFP S.e.) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228049">K3228049 </a> <br> (mTurqoise S.e.) </li>
 +
</b>
 +
</ul>
 +
</html>]]
 +
</div>

Latest revision as of 02:16, 22 October 2019


pMC_0_4_24_TeLuc (S.e.)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

TeLuc is a triple mutant of NanoLuc. It bypasses the absorption of Chlorophyll A, making it the more suitable reporter for phototrophic organism.

This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from iGEM Marburg 2019. They are specifically chosen for cyanobacteria as they show a different absorption and excitation behaviour compared to other bacterial cells due to their photosystems. Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set should be seen as a recommendation of reporters based on experimental dataand literature. They are either fluorescence reporters that bypass the wavelength at which cyanobacteria absorb and emit light or luminescent reporters that show a greater signal strength over background signal.

Compability

These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.

Results

The Bioluminescence of nanoLuc in comparison to teLuc have been measured in e.Coli.

T--Marburg--Measurement-TeLUC%2BNanoluc.png

Available reporters