Difference between revisions of "Part:BBa K2950004"
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<partinfo>BBa_K2950004 short</partinfo> | <partinfo>BBa_K2950004 short</partinfo> | ||
− | ferredoxinTOL reductase (TodA) is involved in the toluene dioxygenase pathway, the pathway we utilized to produce our aim product urushiol, and is identified in Pseudomonas putida. TodA is responsible for the transfer of two electrons from nicotinamide adenine dinucleotide (NADH) to Rieske-type ferredoxinTOL (TodB). | + | <html> |
+ | <p>ferredoxinTOL reductase (TodA) is involved in the toluene dioxygenase pathway, the pathway we utilized to produce our aim product urushiol, and is identified in Pseudomonas putida. TodA is responsible for the transfer of two electrons from nicotinamide adenine dinucleotide (NADH) to Rieske-type ferredoxinTOL (TodB). TodA is 44 kDa, and is characterized via SDS-PAGE electrophoresis.</p> | ||
+ | <div style="text-align:center"> | ||
+ | <br> | ||
+ | <img src="https://2019.igem.org/wiki/images/b/ba/T--BNDS_China--todA.jpeg" width=600px> | ||
+ | </br> | ||
+ | </div> | ||
+ | </html> | ||
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Latest revision as of 15:55, 21 October 2019
ferredoxinTOL reductase (TodA)
ferredoxinTOL reductase (TodA) is involved in the toluene dioxygenase pathway, the pathway we utilized to produce our aim product urushiol, and is identified in Pseudomonas putida. TodA is responsible for the transfer of two electrons from nicotinamide adenine dinucleotide (NADH) to Rieske-type ferredoxinTOL (TodB). TodA is 44 kDa, and is characterized via SDS-PAGE electrophoresis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1173
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 226
Illegal AgeI site found at 319 - 1000COMPATIBLE WITH RFC[1000]