Difference between revisions of "Part:BBa K3040503"

 
 
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<partinfo>BBa_K3040002 short</partinfo>
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<partinfo>BBa_K3040503 short</partinfo>
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FadR is the repressor protein which takes the responsibility to bind on the specific position on pFadBA promoter
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FadR is one of a regulatory enzyme in fatty acid biosynthesis in E. coli. , which acts as the repressor in the fatty acid metabolism system. In the absence of fatty acids, FadR will attach to the gene fragment, and block the entire gene expression.
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3040002 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3040503 SequenceAndFeatures</partinfo>
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We obtain the sequence of FadR directly by cell disruption of E. coli MG1655. After obtaining FadR, we further combine it with the promoter R0010 and a strong RBS B0030.
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3040002 parameters</partinfo>
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<partinfo>BBa_K3040503 parameters</partinfo>
 
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Latest revision as of 11:29, 15 October 2019


Lpp-OmpA-Histag

FadR is one of a regulatory enzyme in fatty acid biosynthesis in E. coli. , which acts as the repressor in the fatty acid metabolism system. In the absence of fatty acids, FadR will attach to the gene fragment, and block the entire gene expression. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

We obtain the sequence of FadR directly by cell disruption of E. coli MG1655. After obtaining FadR, we further combine it with the promoter R0010 and a strong RBS B0030.