Difference between revisions of "Part:BBa K3102015"
 
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HLYA signal peptide found in the C-terminal signal sequence of alpha-hemolysin. Used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria [Nicaud J. M. et al., 1986; Stanley P. et al., 1991]. Unlike Type II signal peptides, fusion of the HlyA signal peptide to the target protein may result in transport of the protein from the cytoplasm to the extracellular medium in a single step.
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__NOTOC__
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<partinfo>BBa_K3102015 short</partinfo>
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HLYA is a signal peptide found in the C-terminal signal sequence of alpha-hemolysin. It is used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria [Nicaud J. M. et al., 1986; Stanley P. et al., 1991]. Unlike Type II signal peptides, fusion of the HlyA signal peptide to the target protein result in transport of the protein from the cytoplasm to the extracellular medium in a single step.
  
 
For our project, this protein is used to secrete both CAE and EG1 proteins by E.coli.
 
For our project, this protein is used to secrete both CAE and EG1 proteins by E.coli.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3102015 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3102015 parameters</partinfo>
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Latest revision as of 10:54, 16 October 2019


hlyA (Hemolysin A)

HLYA is a signal peptide found in the C-terminal signal sequence of alpha-hemolysin. It is used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria [Nicaud J. M. et al., 1986; Stanley P. et al., 1991]. Unlike Type II signal peptides, fusion of the HlyA signal peptide to the target protein result in transport of the protein from the cytoplasm to the extracellular medium in a single step.

For our project, this protein is used to secrete both CAE and EG1 proteins by E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 112
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 112
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 112
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 112
  • 1000
    COMPATIBLE WITH RFC[1000]