Difference between revisions of "Part:BBa K3110003"

 
 
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__NOTOC__
 
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<partinfo>BBa_K3110003 short</partinfo>
 
<partinfo>BBa_K3110003 short</partinfo>
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This is a permease that allows the entry of Lactate into E. coli.
 
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K3110003 parameters</partinfo>
 
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<h1> Usage and Biology</h1>
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This is a combination of lldP (L-Lactate permease), lldR (the regulatory element) and lldD (L-Lactate dehydrogenase). Our intention of using this part to have control over the detection threshold of L-Lactate by the lldPRD regulatory region.

Latest revision as of 10:45, 21 October 2019


lldP + lldR + lldD

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3597
    Illegal AgeI site found at 411
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1471
    Illegal AgeI site found at 1483
    Illegal AgeI site found at 2229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3034


Usage and Biology

This is a combination of lldP (L-Lactate permease), lldR (the regulatory element) and lldD (L-Lactate dehydrogenase). Our intention of using this part to have control over the detection threshold of L-Lactate by the lldPRD regulatory region.