Difference between revisions of "Part:BBa K3110002"

 
 
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lldR is a regulatory protein of the lldPRD operon and has a dual role of activator and repressor. lldD is L-Lactate dehydrogenase and functions by cleaving L-Lactate to Pyruvate. Using a combination of these genes, we intend to regulate the threshold of detection of Lactate by lldPRD regulatory region.
 
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K3110002 parameters</partinfo>
 
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<h1> Usage and Biology</h1>
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lldP is L-Lactate Permease which permits the entry of L-Lactate inside the bacterial cell. lldD is L-Lactate dehydrogenase and functions by cleaving L-Lactate to Pyruvate. Using a combination of these genes, we intend to regulate the threshold of detection of Lactate by lldPRD regulatory region.

Latest revision as of 10:44, 21 October 2019


lldP+lldD

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2805
    Illegal AgeI site found at 411
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1471
    Illegal AgeI site found at 1483
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2242


Usage and Biology

lldP is L-Lactate Permease which permits the entry of L-Lactate inside the bacterial cell. lldD is L-Lactate dehydrogenase and functions by cleaving L-Lactate to Pyruvate. Using a combination of these genes, we intend to regulate the threshold of detection of Lactate by lldPRD regulatory region.