Difference between revisions of "Part:BBa K3253003:Design"

 
 
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===Design Notes===
 
===Design Notes===
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We add extra restriction site in order to make it be successfully cut and transferred
+
This sequence contains multiple restriction site that there can be wrong restriction cutting. Therefore, we add extra restriction site in order to make it be successfully cut and transferred.
  
  
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===Source===
 
===Source===
  
−
This gene comes from the genomic sequence of red chicken-feet cotton, and it is synthesized by the IGEM official.
+
This gene comes from the genomic sequence of red chicken-feet cotton, and it is synthesized by the IGEM official. The gene is often overexpressed in the plant in the conditions in which the plant receives mechanical damage.
  
 
===References===
 
===References===
 +
 +
Ma, Yin-Ping, et al. “Cloning and Functional Analysis of Chitinase Gene GbCHI from Sea-Island Cotton (Gossypium Barbadense).” Hereditas (Beijing), vol. 34, no. 2, 2012, pp. 240–247., doi:10.3724/sp.j.1005.2012.00240.
 +
 +
Liang Luo. Functional study of cotton Chitinase gene Ghchi6 on the  resistance to aphid [D].长江大学,2017.

Latest revision as of 14:48, 20 October 2019


Ghchi 6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence contains multiple restriction site that there can be wrong restriction cutting. Therefore, we add extra restriction site in order to make it be successfully cut and transferred.


Source

This gene comes from the genomic sequence of red chicken-feet cotton, and it is synthesized by the IGEM official. The gene is often overexpressed in the plant in the conditions in which the plant receives mechanical damage.

References

Ma, Yin-Ping, et al. “Cloning and Functional Analysis of Chitinase Gene GbCHI from Sea-Island Cotton (Gossypium Barbadense).” Hereditas (Beijing), vol. 34, no. 2, 2012, pp. 240–247., doi:10.3724/sp.j.1005.2012.00240.

Liang Luo. Functional study of cotton Chitinase gene Ghchi6 on the resistance to aphid [D].长江大学,2017.