Difference between revisions of "Part:BBa K3110000:Design"

 
(→‎Design Notes)
 
(4 intermediate revisions by 2 users not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The gene had an EcoRI site, but we couldn't eliminate it.
+
We took the sequence that is present in wild type E.coli (from ensemble) and codon optimized it using Twist Bioscience codon optimized tool to make it synthesis compatible and remove an in between EcoRI site(as we used this enzyme in cloning purpose).
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
lldD is of Genomic origin and will be joined with Promoter RBS and Terminator using SOEing (Spliced Overlap Extension) PCR.
+
The sequence was synthesized by Twist Biosciences.
  
 
===References===
 
===References===

Latest revision as of 20:53, 21 October 2019


lldD (L-Lactate Dehydrogenase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1149
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 586


Design Notes

We took the sequence that is present in wild type E.coli (from ensemble) and codon optimized it using Twist Bioscience codon optimized tool to make it synthesis compatible and remove an in between EcoRI site(as we used this enzyme in cloning purpose).

Source

The sequence was synthesized by Twist Biosciences.

References