Difference between revisions of "Part:BBa K3279001:Design"

 
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===References===
 
===References===
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Lang, H. P., Cogdell, R. J., Gardiner, A. T. & Hunter, C. N. Early steps in carotenoid biosynthesis: sequences and transcriptional analysis of the crtI and crtB genes of Rhodobacter sphaeroides and overexpression and reactivation of crtI in Escherichia coli and R. sphaeroides. Journal of bacteriology 176, 3859-3869, doi:10.1128/jb.176.13.3859-3869.1994 (1994).

Latest revision as of 04:50, 14 October 2019


Phytoene Synthase (CrtB) from Rhodobacter sphaeroides


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 245
    Illegal NotI site found at 968
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1025
    Illegal XhoI site found at 334
    Illegal XhoI site found at 1006
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 264
    Illegal NgoMIV site found at 722
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primary concern was BioBrick compatibility so the illegal sites in RFC[10] did not appear in the sequence. And the sequence was finally designed to be linked with CrtE and CrtI using overlap PCR.


Source

This part comes from Rhodobacter sphaeroides's genomic sequence.

References

Lang, H. P., Cogdell, R. J., Gardiner, A. T. & Hunter, C. N. Early steps in carotenoid biosynthesis: sequences and transcriptional analysis of the crtI and crtB genes of Rhodobacter sphaeroides and overexpression and reactivation of crtI in Escherichia coli and R. sphaeroides. Journal of bacteriology 176, 3859-3869, doi:10.1128/jb.176.13.3859-3869.1994 (1994).