Difference between revisions of "Part:BBa K143033:Design"
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<partinfo>BBa_K143033 short</partinfo> | <partinfo>BBa_K143033 short</partinfo> | ||
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<partinfo>BBa_K143033 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143033 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks | + | LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks an Lva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on ''B.subtilis''. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (<bbpart>BBa_K143015</bbpart>) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene. |
+ | |||
+ | ====Template==== | ||
+ | |||
+ | ''B. subtilis'' integration plasmid pDR111<cite>#1</cite> | ||
+ | |||
+ | ====Forward Primer==== | ||
+ | |||
+ | 5'- GCT CTA GAT GAA ACC AGT AAC GTT ATA CGA TG - 3' | ||
+ | |||
+ | ====Reverse Primer==== | ||
+ | |||
+ | 5'- GGA CTA GTA TTA TTA CTG CCC GCT TTC CAG TC -3' | ||
===Source=== | ===Source=== | ||
− | The LacI gene was cloned from the ''B. subtilis'' shuttle vector pDR111 using Pfu DNA polymerase PCR | + | The LacI gene was cloned from the ''B. subtilis'' shuttle vector pDR111 using Pfu DNA polymerase PCR. |
===References=== | ===References=== | ||
<biblio> | <biblio> | ||
− | #1 pmid= | + | #1 pmid=12562810 |
</biblio> | </biblio> |
Latest revision as of 00:22, 29 October 2008
LacI (Lva-, N-terminal deletion) regulatory protein
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
LacI was located in the sequence of the B. subtilis shuttle vector pDR111. This version of LacI lacks an Lva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on B.subtilis. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (BBa_K143015) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene.
Template
B. subtilis integration plasmid pDR111#1
Forward Primer
5'- GCT CTA GAT GAA ACC AGT AAC GTT ATA CGA TG - 3'
Reverse Primer
5'- GGA CTA GTA TTA TTA CTG CCC GCT TTC CAG TC -3'
Source
The LacI gene was cloned from the B. subtilis shuttle vector pDR111 using Pfu DNA polymerase PCR.
References
<biblio>
- 1 pmid=12562810
</biblio>