Difference between revisions of "Part:BBa K143021:Design"

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<partinfo>BBa_K143021 short</partinfo>
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===<big>SpoVG Ribosome Binding Site (RBS) for ''B. subtilis''</big>===
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<partinfo>BBa_K143021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143021 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.  
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In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
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===Source===
 
===Source===

Latest revision as of 13:46, 18 September 2008

SpoVG Ribosome Binding Site (RBS) for B. subtilis



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.

Source

The sequence was taken from a previous research paper [1] and was constructed by Geneart.

References

<biblio>

  1. 1 pmid=9669336

</biblio>