Difference between revisions of "Part:BBa K3102036:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We mutated in the MtrC sequence with a Guanine into Cysteine and a Guanine into Adenine at the 1795bp and 1798bp position (G1795C & G1798A), respectively, because of the PstI enzyme (BBa_K3102020). | |
| + | ===Source=== | ||
| + | This sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1627 | ||
| − | + | Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry. | |
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===References=== | ===References=== | ||
Kouzuma A, Kasai T, Hirose A, Watanabe K. Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells. Front Microbiol. 2015;6(JUN):1–11. | Kouzuma A, Kasai T, Hirose A, Watanabe K. Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells. Front Microbiol. 2015;6(JUN):1–11. | ||
Latest revision as of 09:52, 16 October 2019
T7-RBS-MTRC-Ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 270
Design Notes
We mutated in the MtrC sequence with a Guanine into Cysteine and a Guanine into Adenine at the 1795bp and 1798bp position (G1795C & G1798A), respectively, because of the PstI enzyme (BBa_K3102020).
Source
This sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1627
Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
References
Kouzuma A, Kasai T, Hirose A, Watanabe K. Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells. Front Microbiol. 2015;6(JUN):1–11.