Difference between revisions of "Part:BBa K3102035:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | In the DLD-II operon, we mutated a Cysteine into Thymine and a Guanine into Adenine at the 428bp and 433bp position (C428T & G433A), respectively, because of the PstI enzyme. | ||
| + | We also mutated a Adenine into Guanine and a Cysteine into Thymine at the 2799bp and 2802bp position (A2799G & C2802T), respectively, because of the EcoRI enzyme. (BBa_K3102019) | ||
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Kouzuma A, Kasai T, Hirose A, Watanabe K. "Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells". Front Microbiol. 2015;6(JUN):1–11. | ||
Latest revision as of 09:58, 16 October 2019
T7-RBS-DLDII Operon-Ter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4365
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In the DLD-II operon, we mutated a Cysteine into Thymine and a Guanine into Adenine at the 428bp and 433bp position (C428T & G433A), respectively, because of the PstI enzyme. We also mutated a Adenine into Guanine and a Cysteine into Thymine at the 2799bp and 2802bp position (A2799G & C2802T), respectively, because of the EcoRI enzyme. (BBa_K3102019)
Source
This sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1405-MONOMER#tab=TU
Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
References
Kouzuma A, Kasai T, Hirose A, Watanabe K. "Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells". Front Microbiol. 2015;6(JUN):1–11.