Difference between revisions of "Part:BBa K143033"

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<partinfo>BBa_K143033 short</partinfo>
 
<partinfo>BBa_K143033 short</partinfo>
  
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LacI is a regulatory protein responsible for the repression of many catabolite genes. Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (reppresors). Some repressor proteins can be inactivted however by addition of an inducer, such as IPTG or certain sugars.
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LacI if the regulator protein for the lactose operon in ''E.coli'' and the hyper-spank protein of ''B. subtilis''<cite>#1</cite>(<bbpart>BBaK143015</bbpart>) and is responsible for ensuring that in the absence of lactose (or IPTG) that there is no expression trough these promoter. LacI is not endogenous to ''B. subtilis'', so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes.  
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Transcriptional regulator proteins generally regulate trancription by binding to specific operator sequences around the transcription start site. These proteins can affect transcription positively (activators) or negatively (repressors). In addition, many of these regulatory proteins can be regulated themselves by the presence of chemicals and compounds, such as the inducers IPTG.  
  
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This version of LacI lacks a Lva degradation tag and has a small(3 amino acid) N-terminal deletion relative to the current registry LacI (<bbpart>BBa_C0012</bbpart)> and is derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with ''B. subtilis'' though both forms are found in ''E.coli'' (in differing strains).
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LacI is the regulator protein for the lactose operon in ''E. coli'' and the hyper-spank promoter of ''B. subtilis'' <cite>#1</cite> (<bbpart>BBa_K143015</bbpart>) and is responsible for ensuring there is no expression through these promoters in the absence of lactose (or IPTG). LacI is not endogenous to ''B. subtilis'' so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes.  
  
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LacI was used in conjunction with the '''Hyper-spank promoter''' (<bbpart>BBa_K143015<bbpart>) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output
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This version of LacI lacks an Lva degradation tag and has a small (3 amino acid) N-terminal deletion relative to the current registry LacI (<bbpart>BBa_C0012</bbpart>) and its derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with ''B. subtilis'' though both forms are found in ''E. coli'' (in differing strains).
  
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LacI can be used in conjunction with the '''lac operon promoter''' (<bbpart>BBa_K143015</bbpart>), where the LacI will act as a receiver for an IPTG input to result in a '''polymerases per second''' (PoPS) output.
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''<big>Sequence and Features</big>'''</span>
 
<partinfo>BBa_K143033 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143033 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K143033 parameters</partinfo>
 
<partinfo>BBa_K143033 parameters</partinfo>
 
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===References===
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<biblio>
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#1 pmid=16166525
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</biblio>

Latest revision as of 14:16, 7 October 2008

LacI (Lva-, N-terminal deletion) regulatory protein


Transcriptional regulator proteins generally regulate trancription by binding to specific operator sequences around the transcription start site. These proteins can affect transcription positively (activators) or negatively (repressors). In addition, many of these regulatory proteins can be regulated themselves by the presence of chemicals and compounds, such as the inducers IPTG.

LacI is the regulator protein for the lactose operon in E. coli and the hyper-spank promoter of B. subtilis #1 (BBa_K143015) and is responsible for ensuring there is no expression through these promoters in the absence of lactose (or IPTG). LacI is not endogenous to B. subtilis so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes.

This version of LacI lacks an Lva degradation tag and has a small (3 amino acid) N-terminal deletion relative to the current registry LacI (BBa_C0012) and its derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with B. subtilis though both forms are found in E. coli (in differing strains).

LacI can be used in conjunction with the lac operon promoter (BBa_K143015), where the LacI will act as a receiver for an IPTG input to result in a polymerases per second (PoPS) output.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

<biblio>

  1. 1 pmid=16166525

</biblio>