Difference between revisions of "Part:BBa K143031"

Latest revision as of 13:47, 18 September 2008

Aad9 Spectinomycin Resistance Gene

Aad9 is the spectinomycin resistance gene from Enterococcus faecalis#1. Expression in a host confers resistance to spectinomycin at a concentration of 100μg/μl and has been used in a variety of vectors for both B. subtilis and E. coli including pDP870#2, pCOMT-Kan#3 and pIEF16s#4

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 488

References

1 pmid=1659306
2 pmid=16997985
3 pmid=15060042
4 pmid=16714443

</biblio>

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K143031 short</partinfo>
-Aad9 is the spectinomycin resistance gene from ''Enterococcus faecalis''<cite>#1</cite>. Expression in a host confers resistance to spectinomycin at a concentration of 100&#956;g/&#956;l and has been used in a variety of vectors for both ''B. subtilis'' and ''E. coli'' including pDP870<cite>#2</cite>, pCOMT-Kan<cite>#3</cite> and pIEF16s<cite>#4</cite>
+<br>
+Aad9 is the spectinomycin resistance gene from ''Enterococcus faecalis''<cite>#1</cite>. Expression in a host confers resistance to spectinomycin at a concentration of 100&#956;g/&#956;l and has been used in a variety of vectors for both ''B. subtilis'' and ''E. coli'' including pDP870<cite>#2</cite>, pCOMT-Kan<cite>#3</cite> and pIEF16s<cite>#4</cite>
+<br>
+<br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
@@ Line 12: / Line 15: @@
-====References====
+<!-- Uncomment this to enable Functional Parameter display
+===Functional Parameters===
+<partinfo>BBa_K143031 parameters</partinfo>
+<!-- -->
+===References===
 <biblio>
@@ Line 20: / Line 27: @@
 #4 pmid=16714443
 </biblio>
-<!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
-<partinfo>BBa_K143031 parameters</partinfo>
-<!-- -->