Difference between revisions of "Part:BBa K3117005:Design"

Latest revision as of 23:09, 21 October 2019

6xHis-Tag

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

We considered which tag can be used for the purification of our protein. Choosing the histidine-tag, we thought about how many repeats of histidine are useful for our project and chose the 6xHis tag.

Source

This part comes from genomic sequence.

References

1. Booth, William T.; Schlachter, Caleb R.; Pote, Swanandi; Ussin, Nikita; Mank, Nicholas J.; Klapper, Vincent et al. (2018): Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability.

Revision as of 15:08, 13 October 2019 (view source) Registry (Talk \| contribs)		Latest revision as of 23:09, 21 October 2019 (view source) Lena Schorr (Talk \| contribs) (→‎References)
Line 16:		Line 16:

	===References===		===References===
		+
		+	1. Booth, William T.; Schlachter, Caleb R.; Pote, Swanandi; Ussin, Nikita; Mank, Nicholas J.; Klapper, Vincent et al. (2018): Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability.