Difference between revisions of "Part:BBa K3117005"
Lena Schorr (Talk | contribs) |
|||
(3 intermediate revisions by 2 users not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K3117005 short</partinfo> | <partinfo>BBa_K3117005 short</partinfo> | ||
− | + | This part contains six times Histidin (His), which can be used for protein purification. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns. Furthermore, established anti-His antibodies can bind to the 6xHis-tag. This can be used for FACS measurements or protein detection (Booth et al. 2018). | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + |
Latest revision as of 23:07, 21 October 2019
6xHis-Tag
This part contains six times Histidin (His), which can be used for protein purification. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns. Furthermore, established anti-His antibodies can bind to the 6xHis-tag. This can be used for FACS measurements or protein detection (Booth et al. 2018).