Difference between revisions of "Part:BBa K3190103:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The coding sequence of the GPER was fused with the nucleotides for the linker and superfolded GFP in the C-terminus (GPER-Li-sfGFP) to carry out colocalisation assay and characterise the expression and proper alignment of the receptor in the intercellular organelles.
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The coding sequence of the GPER was fused with the nucleotides for the linker and superfolded GFP in the C-terminus (GPER-Li-sfGFP) to carry out localisation assay and characterise the expression and proper alignment of the receptor in the intercellular organelles.
  
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The linker was added in the primer design.
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Linker sequence: GGAGGTGGCTCTGGTGGTGGATCA
  
  
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===References===
 
===References===
 +
<ul>
 +
  <li>Revankar, C. M. (2005). A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling. <i>Science, 307</i>(5715), 1625–1630. doi: 10.1126/science.1106943</li>
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  <li>Ncbi.nlm.nih.gov. (2019). G-protein coupled estrogen receptor 1 [Homo sapiens] - Protein - NCBI Reference Sequence: NP_001091671.1. [online] Available at: https://www.ncbi.nlm.nih.gov/protein/NP_001091671.1?report=genpept [Accessed 18 Oct. 2019]. </li>
 +
  <li>Uniprot.org. (2019). GPER1 - G-protein coupled estrogen receptor 1 - Homo sapiens (Human) - GPER1 gene & protein. [online] Available at: https://www.uniprot.org/uniprot/Q99527 [Accessed 18 Oct. 2019].</li>
 +
</ul>

Latest revision as of 15:37, 18 October 2019


G protein-coupled estrogen receptor (GPER) CDS with Linker-superfolder GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 750
    Illegal SapI.rc site found at 1162


Design Notes

The coding sequence of the GPER was fused with the nucleotides for the linker and superfolded GFP in the C-terminus (GPER-Li-sfGFP) to carry out localisation assay and characterise the expression and proper alignment of the receptor in the intercellular organelles.

The linker was added in the primer design.

Linker sequence: GGAGGTGGCTCTGGTGGTGGATCA


Source

Homo sapiens

References

  • Revankar, C. M. (2005). A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling. Science, 307(5715), 1625–1630. doi: 10.1126/science.1106943
  • Ncbi.nlm.nih.gov. (2019). G-protein coupled estrogen receptor 1 [Homo sapiens] - Protein - NCBI Reference Sequence: NP_001091671.1. [online] Available at: https://www.ncbi.nlm.nih.gov/protein/NP_001091671.1?report=genpept [Accessed 18 Oct. 2019].
  • Uniprot.org. (2019). GPER1 - G-protein coupled estrogen receptor 1 - Homo sapiens (Human) - GPER1 gene & protein. [online] Available at: https://www.uniprot.org/uniprot/Q99527 [Accessed 18 Oct. 2019].