Difference between revisions of "Part:BBa K103004"

Latest revision as of 23:01, 28 October 2008

protein Z_SPA-1

Artificially created strong interactor of B domain of Staphylococcal protein A ( BBa_K103003). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A [http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum].

Z_SPA-1-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris)
We have used Z_SPA-1 protein fusions with BBa_K103019 or BBa_K103020 as a prey in our 'hunter and prey' selection system
This part uses our NdeI/SacI/BamHI fusion cloning substandard

Source: [http://www.pdb.org/pdb/explore.do?structureId=2B88 PDB]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K103004 short</partinfo>
-Artificially created strong interactor of protein A (BBa_K103003)
+{|
+|
-<!-- Add more about the biology of this part here
+Artificially created strong interactor of B domain of Staphylococcal protein A ([[Part:BBa_K103003 | BBa_K103003]]). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A [http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum].
-===Usage and Biology===
+*Z<sub>SPA-1</sub>-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris)
+*We have used Z<sub>SPA-1</sub> protein fusions with [[Part:BBa_K103019 | BBa_K103019]] or [[Part:BBa_K103020 | BBa_K103020]] as a prey in our 'hunter and prey' selection system
+*This part uses our NdeI/SacI/BamHI fusion cloning substandard
+|[[Image:Z_spa.jpg]]
+Source: [http://www.pdb.org/pdb/explore.do?structureId=2B88 PDB]
+|}
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
@@ Line 13: / Line 17: @@
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K103004 parameters</partinfo>
 <!-- -->