Difference between revisions of "Part:BBa K2984005"
 
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The Level 1 plasmid “L1a” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA).
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<partinfo>BBa_K2984005 short</partinfo>
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes <i>BsaI</i> (GGAG/CGCT) and <i>BpiI</i> (GCAA/ACTA).
 
In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.
 
In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2984005 SequenceAndFeatures</partinfo>

Latest revision as of 22:10, 19 October 2019

Level 1 b backbone with GoldenGate Fusionsite

This vector is a part of the Chlamy-HUB-Collection. The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA). In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 729
    Illegal XhoI site found at 721
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal AgeI site found at 1535
    Illegal AgeI site found at 1647
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1823
    Illegal BsaI.rc site found at 752