Difference between revisions of "Part:BBa K2923003"
 
(5 intermediate revisions by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2923003 short</partinfo>
 
<partinfo>BBa_K2923003 short</partinfo>
  
−
An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for the guanine or guanosine ligand.  
+
An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for the guanine or guanosine ligand. It is composed of 3 different parts: a ribozyme module, a communication module and an aptamer module.
 +
 
 +
 
 +
[[Image: Gua active et inactive.jpg|300px|thumb|center|Figure 1: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.]
 +
]]
 +
 
 
This aptazyme is an ON-switches type of aptazyme.  Its catalytic activity (self-cleavage) requires the presence of ligand.
 
This aptazyme is an ON-switches type of aptazyme.  Its catalytic activity (self-cleavage) requires the presence of ligand.
 +
 +
[[Image: Aptazyme process.jpg|500px|thumb|center| Figure 2: Schematic graphic of Aptazyme switch-on catalytic activity]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:00, 20 October 2019


Guanine actif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for the guanine or guanosine ligand. It is composed of 3 different parts: a ribozyme module, a communication module and an aptamer module.


Figure 1: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.]

This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand.

Figure 2: Schematic graphic of Aptazyme switch-on catalytic activity

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]