Difference between revisions of "Part:BBa K2923002"
 
(9 intermediate revisions by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2923002 short</partinfo>
 
<partinfo>BBa_K2923002 short</partinfo>
  
−
An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. this aptazyme is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. It is composed in 3 different part, a twister ribozyme module, a communication module, and an aptamer module This sequence correspond to inactive aptazyme, this means that the ribozyme part is mutated and the aptazyme lost its capacity to cleave itself. iGEM Strasbourg 2019 used it as a negative control for the part: <BBa_K2923001>
+
An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. this aptazyme is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. It is composed of 3 different parts: a twister ribozyme module, a communication module and an aptamer module. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: <partinfo>BBa_K2923001</partinfo>
−
 
+
  
 +
[[Image: BasicPartsimg1.jpg|400px|thumb|center|Figure 1: Theophylline aptazyme secondary structure. The Shine-Dalgarno (SD) and the anti-SD are shown in light blue and grey, respectively. The inactivating mutation is highlighted in yellow. The cleavage site is indicated with a grey arrow. The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:00, 20 October 2019


Theophylline inactif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. this aptazyme is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. It is composed of 3 different parts: a twister ribozyme module, a communication module and an aptamer module. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923001

Figure 1: Theophylline aptazyme secondary structure. The Shine-Dalgarno (SD) and the anti-SD are shown in light blue and grey, respectively. The inactivating mutation is highlighted in yellow. The cleavage site is indicated with a grey arrow. The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]