Difference between revisions of "Part:BBa K2923002:Design"
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K2923007 short</partinfo>
+
<partinfo>BBa_K2923002 short</partinfo>
  
−
<partinfo>BBa_K2923007 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2923002 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
−
=Aptazyme is composed of three different part :
+
Aptazyme is composed of three different parts: Theo5 as a communication module, aptamer module specific to theophylline and P1 as a twister ribozyme mutated in the 14 and 15 positions (GT→TG) to inhibit the RNA cleavage activity. The no highlighted sequences correspond to the riboswitch module.
−
=Theo5 as a communication module,
+
−
=P1 as a twister ribozyme mutated in the 14 an 15 position to inhibit the RNA cleavage activity of the ribozyme part
+
−
=Aptamer module specific to theophylline.
+
−
 
+
−
 
+
  
  

Latest revision as of 11:00, 13 October 2019

Theophylline inactif aptazyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Aptazyme is composed of three different parts: Theo5 as a communication module, aptamer module specific to theophylline and P1 as a twister ribozyme mutated in the 14 and 15 positions (GT→TG) to inhibit the RNA cleavage activity. The no highlighted sequences correspond to the riboswitch module.


Source

Felletti, M., Stifel, J., Wurmthaler, L.A., Geiger, S., and Hartig, J.S. (2016). Twister ribozymes as highly versatile expression platforms for artificial riboswitches. Nature Communications 7