Difference between revisions of "Part:BBa K3140001:Design"

 
(→‎References)
 
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
NA
 
−
 
  
 +
This part was intended for use in pUS381 for initial analysis of protein expression and final expression. To achieve this, the part was ordered in a gBlock flanked by BamHI and XbaI recognition sequences to enable traditional restriction cloning into pUS381, along with the consensus Shine-Dalgarno sequence as an RBS upstream of the coding sequence.
  
 
===Source===
 
===Source===
  
−
From Psilocybe cubensis
+
The native sequence for the putative cytochrome P450 monooxygenase PsiH (''Psilocybe cubensis'') was obtained from NCBI: GenBank accession [https://www.ncbi.nlm.nih.gov/nuccore/MF000993.1 MF000993.1]
  
 
===References===
 
===References===
 +
 +
'''1.''' Fricke, J., Blei, F. & Hoffmeister, D. Enzymatic Synthesis of Psilocybin. ''Angew Chem Int Ed Engl'' '''56''', 12352-12355 (2017).

Latest revision as of 06:43, 15 October 2019


PsiH - Cytochrome P450 monooxygenase from Psilocybe cubensis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was intended for use in pUS381 for initial analysis of protein expression and final expression. To achieve this, the part was ordered in a gBlock flanked by BamHI and XbaI recognition sequences to enable traditional restriction cloning into pUS381, along with the consensus Shine-Dalgarno sequence as an RBS upstream of the coding sequence.

Source

The native sequence for the putative cytochrome P450 monooxygenase PsiH (Psilocybe cubensis) was obtained from NCBI: GenBank accession MF000993.1

References

1. Fricke, J., Blei, F. & Hoffmeister, D. Enzymatic Synthesis of Psilocybin. Angew Chem Int Ed Engl 56, 12352-12355 (2017).