Difference between revisions of "Part:BBa K3205001"
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<partinfo>BBa_K3205001 short</partinfo> | <partinfo>BBa_K3205001 short</partinfo> | ||
− | Pare is a AreR-regulated σ54-dependent promoter in Acinetobacter sp. Strain ADP1 and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate. | + | <i>Pare</i> is a AreR-regulated σ54-dependent promoter in <i>Acinetobacter</i> sp. Strain ADP1. <i>are</i> operon and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate. |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol or other aromatic compounds. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream gene transcription. Combining this element with the AreR gene can form a complete gene expression regulation device for signaling pathways requiring benzyl alcohol induction. | + | AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the <i>areCBA</i> genes in <i>Acinetobacter</i> sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the <i>Pare</i> promoter after activated by benzyl alcohol or other aromatic compounds. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream gene transcription. Combining this element with the AreR gene can form a complete gene expression regulation device for signaling pathways requiring benzyl alcohol induction. |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 13:01, 16 October 2019
Pare promoter region
Pare is a AreR-regulated σ54-dependent promoter in Acinetobacter sp. Strain ADP1. are operon and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.
Usage and Biology
AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol or other aromatic compounds. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream gene transcription. Combining this element with the AreR gene can form a complete gene expression regulation device for signaling pathways requiring benzyl alcohol induction. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
For more details, please click the link below.
https://parts.igem.org/Part:BBa_K3205000