Difference between revisions of "Part:BBa K2983060"

Latest revision as of 17:37, 21 October 2019

RedStar2

RedStar2 is a derivative of the Red Fluorescent Protein from Discosoma sp. with improved features in terms of intrinsic brightness and speed of maturation [1]. The protein is tetrameric and contains 15 mutations compared to wild-type (Uniprot Q9U6Y8) : deltaR2, K5E, N6D, K9T, R17K, H41T, N42Q, V44A, V96I, F124L, A145P, M182K, P186Q, T202I, T217A.

This part is derived from coding sequence of RedStar2 used by Larroude et al. [2].

Usage and Biology

Applications are various: Fusions, promoter & secretion studies, FRET and other quantitative imaging techniques.

References

[1] Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M. A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast (2004) 21, 947-962.

[2] Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T. A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology. Microb Biotechnol (2019) 12, 1249– 1259.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2983060 short</partinfo>
-RedStar2
+RedStar2 is a derivative of the Red Fluorescent Protein from ''Discosoma sp.'' with improved features in terms of intrinsic brightness and speed of maturation [1]. The protein is tetrameric and contains 15 mutations compared to wild-type (Uniprot Q9U6Y8) : deltaR2, K5E, N6D, K9T, R17K, H41T, N42Q, V44A, V96I, F124L, A145P, M182K, P186Q, T202I, T217A.
+This part is derived from coding sequence of RedStar2 used by Larroude ''et al.'' [2].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Applications are various: Fusions, promoter & secretion studies, FRET and other quantitative imaging techniques.
+===References===
+[1] Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M. A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast (2004) 21, 947-962.
+[2] Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T. A modular Golden Gate toolkit for ''Yarrowia lipolytica'' synthetic biology. Microb Biotechnol (2019) 12, 1249– 1259.
 <!-- -->