Difference between revisions of "Part:BBa K3102014"

Latest revision as of 15:11, 14 October 2019

LldP (L-lactate permease)

E.coli, lldP is an inner membrane permease involved in the uptake of L-lactate, D-lactate and glycolate [Dong et al., 1993; Nunez et al., 2002]. The lldP gene is located in a lactate-inducible operon with the lldD and lldR genes encoding a lactate dehydrogenase and a regulatory protein, respectively. By overexpressing a plasmid carrying this enzyme, the bacteria is able to uptake twice the amount of these three substrates, L-lactate, D-lactate and glycolate.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 275
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 411
Illegal AgeI site found at 874
Illegal AgeI site found at 1312
Illegal AgeI site found at 1471
Illegal AgeI site found at 1483
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3102014 short</partinfo>
-E.coli, lldP is an inner membrane permease that functions in the uptake of L-lactate, D-lactate and glycolate [Dong et al., 1993; Nunez et al., 2002]. The lldP gene is located in a lactate-inducible operon with the lldD and lldR genes encoding a lactate dehydrogenase and a regulatory protein, respectively. By overexpressing a plasmid carrying this enzyme, the bacteria is able to uptake the double of these three substrates, L-lactate, D-lactate and glycolate.
+E.coli, lldP is an inner membrane permease involved in the uptake of L-lactate, D-lactate and glycolate [Dong et al., 1993; Nunez et al., 2002]. The lldP gene is located in a lactate-inducible operon with the lldD and lldR genes encoding a lactate dehydrogenase and a regulatory protein, respectively. By overexpressing a plasmid carrying this enzyme, the bacteria is able to uptake twice the amount of these three substrates, L-lactate, D-lactate and glycolate.
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