Difference between revisions of "Part:BBa K2997004"

 
 
(One intermediate revision by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2997004 short</partinfo>
 
<partinfo>BBa_K2997004 short</partinfo>
  
−
Eventually, the positive P. fluorescens 55 clone with our pchR-GFP construct is chosen to undergo the functional test as P. aeruginosa DAP is a BSL2 classified organism, which is not aligned with our aim of having a safe detection device.
+
===Background===
−
 
+
−
The functional test results are depicted below:
+
  
 +
This region is where the eventual protein-<i>p</i>-Cresol complex (when activator protein binds with <i>p</i>-Cresol) will bind onto to promote the expression of the downstream gene (GFP in our case).
  
  
Line 21: Line 20:
 
<partinfo>BBa_K2997004 parameters</partinfo>
 
<partinfo>BBa_K2997004 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===References===
 +
 +
<html>
 +
<br>
 +
<ol type="1">
 +
<li>Cho, Ah Ra, Eun Jin Lim, Yaligara Veeranagouda, and Kyoung Lee* Department of Microbiology, Changwon National University, Changwon-si, Kyongnam 641-773, Korea . Identification of a <i>p</i>-Cresol Degradation Pathway by a GFP-Based Transposon in <i>Pseudomonas</i> and Its Dominant Expression in Colonies</li>
 +
<li>Merike Jo ˜esaar, Eeva Heinaru, Signe Viggor, Eve Vedler & Ain Heinaru Institute of Molecular and Cell Biology, Tartu University, Tartu, Estonia. Diversity of the transcriptional regulation of the pch gene cluster in two indigenous <i>p</i>-Cresol-degradative strains of <i>Pseudomonas fluorescens</i>
 +
</li>
 +
<br>
 +
</html>

Latest revision as of 07:19, 20 October 2019


PchR Protein Binding Range

Background

This region is where the eventual protein-p-Cresol complex (when activator protein binds with p-Cresol) will bind onto to promote the expression of the downstream gene (GFP in our case).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References


  1. Cho, Ah Ra, Eun Jin Lim, Yaligara Veeranagouda, and Kyoung Lee* Department of Microbiology, Changwon National University, Changwon-si, Kyongnam 641-773, Korea . Identification of a p-Cresol Degradation Pathway by a GFP-Based Transposon in Pseudomonas and Its Dominant Expression in Colonies
  2. Merike Jo ˜esaar, Eeva Heinaru, Signe Viggor, Eve Vedler & Ain Heinaru Institute of Molecular and Cell Biology, Tartu University, Tartu, Estonia. Diversity of the transcriptional regulation of the pch gene cluster in two indigenous p-Cresol-degradative strains of Pseudomonas fluorescens