Difference between revisions of "Part:BBa K2924035:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
The sequence is codon-optimized for Synechocystis sp. PCC 6803.
+
The sequence is codon-optimized for Synechocystis sp. PCC 6803. It was provided by Anna Behle<sup>1</sup>.
 +
 
 +
===Source===
 +
The gene was originally found in <i>Aequorea victoria</i>
  
 
===References===
 
===References===
−
Kremers, Gert-Jan, et al. "Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius." Biochemistry 45.21 (2006): 6570-6580.
 
  
 
Behle, Anna, Pia Saake, and Ilka M. Axmann. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.
 
Behle, Anna, Pia Saake, and Ilka M. Axmann. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.
  
 
https://www.fpbase.org/protein/mvenus/
 
https://www.fpbase.org/protein/mvenus/

Latest revision as of 15:54, 21 October 2019


mVenus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon-optimized for Synechocystis sp. PCC 6803. It was provided by Anna Behle1.

Source

The gene was originally found in Aequorea victoria

References

Behle, Anna, Pia Saake, and Ilka M. Axmann. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.

https://www.fpbase.org/protein/mvenus/